Data Tables
Fluorescence Quantum Yield Standards
The most frequently used method of determining the quantum yield of a fluorophore is by comparison with a standard of known quantum yield. The table of quantum yield standards lists dyes that are frequently used as standards in such relative quantum yield measurements.
| Quantum Yield [Q.Y.] Standards | Q.Y.[%] | Conditions for Q.Y. Measurement | Excitation [nm] | Ref. |
|---|---|---|---|---|
| Cy3 | 4 | PBS | 540 | 1 |
| Cy5 | 27 | PBS | 620 | 1 |
| Cresyl Violet | 53 | Methanol | 580 | 2 |
| Fluorescein | 95 | 0.1 M NaOH, 22°C | 496 | 2 |
| POPOP | 97 | Cyclohexane | 300 | 2 |
| Quinine Sulfate | 58 | 0.1 M H2SO4, 22°C | 350 | 2 |
| Rhodamine 101 | 100 | Ethanol | 450 | 3 |
| Rhodamine 6G | 95 | Water | 488 | 3 |
| Rhodamine B | 31 | Water | 514 | 3 |
| Tryptophan | 13 | Water, 20°C | 280 | 2 |
| L-Tyrosine | 14 | Water | 275 | 2 |
PBS = phosphate-buffered saline
POPOP = 1, 4-bis(5-phenyloxazole-2-yl)benzene
References
- R.B. Mujumdar, L.A. Ernst, S.R. Mujumdar, C.J. Lewis, A.S. Waggoner, Cyanine dye labeling reagents: sulfoindocyanine succinimidyl esters. Bioconj Chem 4, 105-111, 1993.
- J.R. Lakowicz, Principles of Fluorescence Spectroscopy, 2nd Ed., Kluwer Academic/Plenum Publishers, New York, London, Moscow, Dordrecht, 1999.
- D. Magde, G.E. Rojas, and P. Seybold, Solvent Dependence of the Fluorescence Lifetimes of Xanthene Dyes. Photochem. Photobiol. 70, 737, 1999.
